Journal: Journal of materials chemistry. B

Article Title: The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition

doi: 10.1039/C7TB03106H

Figure Lengend Snippet: Cartoon depiction of production of four PEG-TMV and four SA-TMV constructs, with varying PEG linker length and SA coverage quantity. TMV shown in gray, PEG linker shown in cyan, and serum albumin shown in red. Conjugation of PEG4 or PEG24 to TMV results in four PEG-TMV constructs: 1′ (short linker, low coverage), 2′ (short linker, high coverage), 3′ (long linker, low coverage), and 4′ (long linker, high coverage). PEG-TMV constructs are reacted with PEG4-conjugated SA to yield four SA-TMV constructs: 1 (short linker, low coverage) as shown in the orange box, 2 (short linker, high coverage) in the blue box, 3 (long linker, low coverage) in the purple box, and 4 (long linker, high coverage) in the green box.

Article Snippet: Dot blots Blots were prepared by spotting 1 μL of 150 μg/mL rabbit polyclonal anti-TMV antibody (custom-made, Pacific Immunology), rabbit polyclonal anti-CPMV antibody (custom-made, Pacific Immunology), rabbit monoclonal anti-PEG antibody ( {"type":"entrez-nucleotide","attrs":{"text":"AB133471","term_id":"62154052","term_text":"AB133471"}} AB133471 , Abcam), or 1:200 diluted mouse plasma on a nitrocellulose membrane, after the nitrocellulose membrane was equilibrated in PBS.

Techniques: Construct, Conjugation Assay

Journal: Journal of materials chemistry. B

Article Title: The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition

doi: 10.1039/C7TB03106H

Figure Lengend Snippet: Physical characteristics of SA coverage and PEG linkers of SA-TMV constructs.

Article Snippet: Dot blots Blots were prepared by spotting 1 μL of 150 μg/mL rabbit polyclonal anti-TMV antibody (custom-made, Pacific Immunology), rabbit polyclonal anti-CPMV antibody (custom-made, Pacific Immunology), rabbit monoclonal anti-PEG antibody ( {"type":"entrez-nucleotide","attrs":{"text":"AB133471","term_id":"62154052","term_text":"AB133471"}} AB133471 , Abcam), or 1:200 diluted mouse plasma on a nitrocellulose membrane, after the nitrocellulose membrane was equilibrated in PBS.

Techniques: Construct, Molecular Weight, Concentration Assay

Journal: Journal of materials chemistry. B

Article Title: The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition

doi: 10.1039/C7TB03106H

Figure Lengend Snippet: Characterization of SA-TMV constructs. A) Representative SDS-PAGE gel. B) Overlaid WBs against TMV (green) and SA (red). C) Negative stain TEM micrographs showing intact SA-TMV particles. The four SA-TMV constructs are: 1 (short linker, low coverage), 2 (short linker, high coverage), 3 (long linker, low coverage), and 4 (long linker, high coverage). Scale bars = 100 nm. D) Schematic showing SA interacting with PEGs on TMV, with the mushroom conformation of 24-mer PEG preventing conjugation. Reactive end denoted with a star.

Article Snippet: Dot blots Blots were prepared by spotting 1 μL of 150 μg/mL rabbit polyclonal anti-TMV antibody (custom-made, Pacific Immunology), rabbit polyclonal anti-CPMV antibody (custom-made, Pacific Immunology), rabbit monoclonal anti-PEG antibody ( {"type":"entrez-nucleotide","attrs":{"text":"AB133471","term_id":"62154052","term_text":"AB133471"}} AB133471 , Abcam), or 1:200 diluted mouse plasma on a nitrocellulose membrane, after the nitrocellulose membrane was equilibrated in PBS.

Techniques: Construct, SDS Page, Staining, Conjugation Assay

Journal: Journal of materials chemistry. B

Article Title: The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition

doi: 10.1039/C7TB03106H

Figure Lengend Snippet: Antibody recognition of SA-TMV and PEG-TMV constructs. A) Dot blots of anti-TMV or anti-CPMV recognition of fluorescent SA-TMV constructs (1-4) and their corresponding PEG-TMV control constructs (1′-4′). B) Densitometry quantification of fluorescent TMV signal corresponding to antibody recognition by anti-TMV antibodies from A. C) Dot blots of anti-TMV or anti-PEG recognition of fluorescent high coverage SA-TMV constructs (2 and 4) and their corresponding PEG-TMV control constructs (2′ and 4′). D) Densitometry quantification of fluorescent TMV signal corresponding to antibody recognition by anti-TMV and anti-PEG antibodies from C.

Article Snippet: Dot blots Blots were prepared by spotting 1 μL of 150 μg/mL rabbit polyclonal anti-TMV antibody (custom-made, Pacific Immunology), rabbit polyclonal anti-CPMV antibody (custom-made, Pacific Immunology), rabbit monoclonal anti-PEG antibody ( {"type":"entrez-nucleotide","attrs":{"text":"AB133471","term_id":"62154052","term_text":"AB133471"}} AB133471 , Abcam), or 1:200 diluted mouse plasma on a nitrocellulose membrane, after the nitrocellulose membrane was equilibrated in PBS.

Techniques: Construct